Coding

Part:BBa_K3904601:Design

Designed by: Karolis Sabutis   Group: iGEM21_Vilnius-Lithuania   (2021-09-19)


Pyruvate, phosphate dikinase (PPDK)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2656
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1149
    Illegal NgoMIV site found at 2409
    Illegal AgeI site found at 2187
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1285
    Illegal SapI.rc site found at 1753


Design Notes

we needed to adapt the nucleotides


Source

entamoeba histolytica genome

References